E. Coli

LacZ

LB Agar- will grow any bacteria

LB/amp agar- contains ampicillin and luriobroth, screens for presence of plasmid in the bacteria LB/amp/ara- arabinose is raw material for the GFP gene to make green fluorescent protein. Gene araC, contains arabinose

Intro: Is is possible to create super bacteria that can glow in the dark and are antibiotic resistant. The purpose of this lab is to use Pglo and insert that into the bacteria. To make them resistant to antibiotics we will use ampicillin to kill off the week ones.

Discussion: The goal of this lab was to determine what solutions would show the most growth with bacteria and pGlo. The group that showed the most growth when held under the UV light was the container with LB/amp/ara. The reason for this is is because it had ampicillin to kill some bacteria, while it also had the arabinose to make it glow in the dark. That way the remaining bacteria would be antibiotic resistant.

Conclusion:  in the end we were able to successfully cultivate pglo in the ecoli.  We were able to see that when we integrated jellyfish DNA into certain cultures of ecoli depending on the environment that we put them in.  The pglo- never had a chance to glow because we never integrated the DNA and the pglo+ were able to glow.  Certain cultures were able to glow more because we added ampicillin to kill off excess bacteria.  Our cultures came out the way that we expected and we were able to cultivate more pglo ecoli than most of the groups in our lab.