Grasshopper Anatomy

Title: Grasshopper

Where it lives/found: found in tropical areas and in wet zones

What does it eat: eat plants. Many are referred to as crop destroyers

How does it breathe: exchange air in their tissues through their trachea 

Include at least one "fun fact" that is unique to the specimen: People eat grasshoppers in certain parts of the world becasue they have tons of protein

Major Internal/External Anatomy

Cray fish

Title: Crayfish

Where it lives/found: live in freshwater areas and also swamps

What does it eat: they eat living and dead animals and plants

How does it breathe: they breathe through feather like gills 

Include at least one "fun fact" that is unique to the specimen: there are no crayfish in africa

Major Internal/External Anatomy

Frog Anatomy

Title: Grass Frog

Where it lives/found: found in Sub Saharan Africa and Egypt

What does it eat: the grass frog feeds on insects and plants

How does it breathe: breathes through its skin and also through gills as a tadpole

Include at least one "fun fact" that is unique to the specimen: they are protected by the Wildlife and Countryside Act of 1981

Major Internal/External Anatomy

Perch Anatomy

Title: Perch

Where it lives/found: if they are truly from the perch species they are found only in freshwater

What does it eat: they eat small fish and insects, crayfish, and occasionally they eat other perch

How does it breathe: they breathe through gills like fish

Include at least one "fun fact" that is unique to the specimen: The Nile perch can reach up to 6 feet 500lbs

Major Internal/External Anatomy

Starfish Anatomy

Starfish Anatomy

Where it lives/found: they are found in the intertidal zone of the ocean floor and they are found in the seabed of the ocean

What does it eat: they feed mostly on benthic invertebrate because they are opportunistic feeders

How does it breathe: uses a hydraulic system to perform gas exchange

Include at least one "fun fact" that is unique to the specimen: Famous character Patricik Star is based on the star fish

Major Internal/External Anatomy

Clam Anatomy

Title: Clam

Where it lives/found: live in both freshwater and marine habitats

What does it eat: they are filter feeders so they eat small molecules of food passed through a system

How does it breathe: they breathe through gills like fish

Include at least one "fun fact" that is unique to the specimen: Moche people of ancient Peru used to worship the clam

Major Internal/External Anatomy

Earthworm Anatomy

Title: Earthworm

Where it lives/found: Dirt. In the Earth. They drown in water

What does it eat: They eat decaying roots and leaves in the soil that gives them their nutrients 

How does it breathe: worms breathe through their skin so the oxygen goes righ to their blood Include at least one "fun fact" that is unique to the specimen: each earthworm is male and female

Major Internal/External Anatomy

Hardy-Weinberg Problems

Restriction Mapping

Introduction:  in a DNA electrophoresis lab you use electric shocks to separate bands of DNA based on there length.  The longer the DNA the less it will go down the gel because of the resistance.  We have the DNA flow from negative end to positive end due to the negative charge of DNA in the phorphorous.  DNA is then able to be identified based on the length of each DNA observed if you are able to match up bands that means you are able to match up people.  This technique is used by law enforcement to identify criminals.  

Discussion: Our lab was a disaster. First off, we ripped our gel in half. Then we put it in the wrong way. So we had no results. For educational purposes we used Mr. Filipek's results as our own. First we examined the first lane and found there were two fragments, meaning it was cut in two places. The one fragment was about 700 long and the second one was about 4700 long. The total distance for each lane had to be about 5400. The second lane had three fragments, meaning it had three cuts. The one fragment was still 700 meaning the remainder had to be a total of 4700. We split that up into 2500 and 2200. The third lane had three cuts again, meaning three fragments. The 700 fragment still stood, we had a fragment that was 4200, and the final 500 was made up by the enzyme hpt1. In the last lane we put all of these results together into one fragment.

Conclusion: The lab is easy to map if you do it right. It helps not to rip the gel in half, and not to load it backwards. Other groups had much more desired results and by looking at those it is easy to see what DNA belonged where. Our results would've been much better if we were more careful with the materials.

E. Coli

LacZ

LB Agar- will grow any bacteria

LB/amp agar- contains ampicillin and luriobroth, screens for presence of plasmid in the bacteria LB/amp/ara- arabinose is raw material for the GFP gene to make green fluorescent protein. Gene araC, contains arabinose

Intro: Is is possible to create super bacteria that can glow in the dark and are antibiotic resistant. The purpose of this lab is to use Pglo and insert that into the bacteria. To make them resistant to antibiotics we will use ampicillin to kill off the week ones.

Discussion: The goal of this lab was to determine what solutions would show the most growth with bacteria and pGlo. The group that showed the most growth when held under the UV light was the container with LB/amp/ara. The reason for this is is because it had ampicillin to kill some bacteria, while it also had the arabinose to make it glow in the dark. That way the remaining bacteria would be antibiotic resistant.

Conclusion:  in the end we were able to successfully cultivate pglo in the ecoli.  We were able to see that when we integrated jellyfish DNA into certain cultures of ecoli depending on the environment that we put them in.  The pglo- never had a chance to glow because we never integrated the DNA and the pglo+ were able to glow.  Certain cultures were able to glow more because we added ampicillin to kill off excess bacteria.  Our cultures came out the way that we expected and we were able to cultivate more pglo ecoli than most of the groups in our lab.  

Strawberries DNA Lab

The goal of our lab was to extract DNA from a strawberry. First what we did was take off the leaves of the strawberry and mash up the strawberry in a plastic bag. Then what we did was add shampoo to the solution in order to seperate the components of the strawberry and denature the proteins. Next we took the liquid solution of shampoo and strawberry and put it in a beaker. After this we added alcohol solution to the top of the beaker and let the alcohol further denature the liquid solution and expose the DNA from the rest of the solution. After this we inserted a small hook into the beaker and spun the hook around in order to catch the DNA.

What is Mitosis?